|
In this study, we report the callus induction from the cormel tissue explants and the subculture methods for further proliferation of the callus in several cultivars of gladiolus. It was possible to induce callus from the cormel tissue explants in the 11 cultivars cultured on MS solid medium containing 10 ㎎/ℓ 2,4-D. The percentages and days of the callus formation in each cultivars were ranged from 21.5 % to 100 % and about 26 days, respectively. External appearance and color of the gladiolus callus was small globular cluster, soft, friable, nonchlorophyllus and light yellow, regardless of cultivar, in MS medium containing 0.5 ㎎/ℓ 2,4-D. The callus was mainly induced from the inner and outer parts in a cormel tissue explant. Among the 11 cultivars, callus subculture in 2,4-D medium was only possible for 4 cultivars ; ´Firebrand´, ´Topaz´, ´True Love´, and ´Spic and Span´. However, the uniform callus could be only maintained and proliferated in ´Topaz´. In the other 3 cultivars, the callus was mixed with somatic embryos or organs. Optimum concentrations of 2,4-D in 1st and 2nd subculture for further proliferation of the ´Topaz´ callus were 0-5 ㎎/ℓ and 0.1-1 ㎎/ℓ, respectively. Callus weight in subculture was the best in 20 ㎎, small size for callus proliferation. Callus growth showed a typical sigmoid curve which shows a lag phase for 2 weeks and reached a stationary phase after 6 weeks in subculture.
|